Abstract
We have previously shown that the inhibition of fibroblast growth factor (FGF) signaling induced endodermal gene expression in the animal cap and caused the expansion of the endodermal mass in Xenopus embryos. However, we still do not know whether or not the alteration of FGF signaling controls embryonic cell fate, or when FGF signal blocking is required for endoderm formation in Xenopus. Here, we show that FGF signal blocking in embryonic cells causes their descendants to move into the endodermal region and to express endodermal genes. It is also interesting that blocking FGF signaling between fertilization and embryonic stage 10.5 promotes endoderm formation, but persistent FGF signaling blocking after stage 10.5 restricts endoderm formation and differentiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Endoderm / drug effects
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Endoderm / embryology
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Endoderm / metabolism*
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Fibroblast Growth Factors / antagonists & inhibitors
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Fibroblast Growth Factors / genetics
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Fibroblast Growth Factors / physiology*
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Gene Expression Regulation, Developmental / drug effects
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In Situ Hybridization
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Pyrroles / administration & dosage
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Pyrroles / pharmacology
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Receptors, Fibroblast Growth Factor / genetics
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Receptors, Fibroblast Growth Factor / physiology
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Reverse Transcriptase Polymerase Chain Reaction
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Signal Transduction / drug effects
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Xenopus Proteins / antagonists & inhibitors
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Xenopus Proteins / genetics
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Xenopus Proteins / physiology*
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Xenopus laevis / embryology
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Xenopus laevis / genetics
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Xenopus laevis / physiology*
Substances
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Pyrroles
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Receptors, Fibroblast Growth Factor
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SU 5402
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Xenopus Proteins
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Fibroblast Growth Factors