Detection of miR-106a in gastric carcinoma and its clinical significance

Clin Chim Acta. 2009 Feb;400(1-2):97-102. doi: 10.1016/j.cca.2008.10.021. Epub 2008 Oct 30.

Abstract

Background: MicroRNAs (miRNAs) play important roles in carcinogenesis. miRNA-106a (miR-106a) has oncogenic activity in humans, and often has altered expression. The clinical significance of miR-106a in the diagnosis of gastric carcinoma is poorly understood.

Methods: The level of miR-106a in 55 gastric carcinoma and 17 non-tumor tissues was quantified by real-time reverse transcriptase-polymerase chain reaction, and the relationship between miR-106a level and clinical and pathological factors was explored.

Results: The level of miR-106a in cancer tissues was significantly higher than that in non-tumor tissues, with an average 1.625-fold increase. miR-106a level was significantly associated with tumor stage, size and differentiation; lymphatic and distant metastasis; and invasion (P<0.01). The altered expression of miR-106a was confirmed in gastric cancer cell lines.

Conclusion: miR-106a may be a potential biomarker in the diagnosis of gastric carcinoma.

MeSH terms

  • Biomarkers / analysis
  • Cell Line, Tumor
  • E2F1 Transcription Factor / genetics
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Male
  • MicroRNAs / analysis*
  • MicroRNAs / genetics
  • Middle Aged
  • Neoplasm Metastasis / genetics
  • Neoplasm Staging
  • Retinoblastoma Protein / genetics
  • Stomach Neoplasms / diagnosis*
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology
  • Up-Regulation

Substances

  • Biomarkers
  • E2F1 Transcription Factor
  • E2F1 protein, human
  • MicroRNAs
  • Retinoblastoma Protein