Trifluoromethyldiazirine: an effective photo-induced cross-linking probe for exploring amyloid formation

Chem Commun (Camb). 2008 Nov 30:(44):5728-30. doi: 10.1039/b813504e. Epub 2008 Sep 30.

Abstract

The separative and analytical power of ion mobility spectrometry-mass spectrometry combined with photo-induced cross-linking of site-specifically incorporated trifluoromethyldiazirine provides a powerful approach towards structural characterisation of amyloid fibrils.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amyloid / chemistry*
  • Amyloid / ultrastructure
  • Amyloid beta-Peptides / chemistry*
  • Amyloid beta-Peptides / metabolism
  • Azirines / chemistry*
  • Cross-Linking Reagents / chemistry*
  • Methane / analogs & derivatives
  • Methane / chemistry
  • Peptides / chemical synthesis
  • Peptides / chemistry
  • Phenylalanine / analogs & derivatives*
  • Phenylalanine / chemical synthesis
  • Phenylalanine / chemistry
  • Protein Structure, Tertiary
  • Spectrometry, Mass, Electrospray Ionization
  • Ultraviolet Rays

Substances

  • Amyloid
  • Amyloid beta-Peptides
  • Azirines
  • Cross-Linking Reagents
  • Peptides
  • trifluoromethyldiazirine-phenylalanine
  • carbene
  • Phenylalanine
  • Methane