A total of 1,101 Chinese healthcare workers were surveyed to determine the perception of institutional support for HIV/AIDS care in China. Multilevel regression models with random effects were used to estimate individual and facility-level effects on healthcare workers' perceived institutional support. Regional workers (at the provincial, city, county levels) reported higher institutional support for HIV/AIDS care than local workers (at the township and village levels). Estimated relationships between perceived institutional support and HIV-related training were greater for the regional than for the local level. Urgent need exists to address the unbalanced healthcare system in China and to consider the policy implications for an effective response to the HIV/AIDS epidemic.