Cardiac damage after lesions of the nucleus tractus solitarii

Am J Physiol Regul Integr Comp Physiol. 2009 Feb;296(2):R272-9. doi: 10.1152/ajpregu.00080.2008. Epub 2008 Nov 19.

Abstract

Humans with central lesions that augment sympathetic nerve activity are predisposed to cardiac arrhythmias, myocardial lesions, and sudden death. Previously, we showed that selectively killing neurons with neurokinin-1 receptors in the nucleus tractus solitarii (NTS) of rats attenuated the baroreflex and, in some animals, led to sudden unexplained death within approximately 2 wk. Interruption of arterial baroreflexes is known to increase sympathetic activity. Here we tested the hypothesis that lesions in the NTS lead to fatal cardiac arrhythmias and myocardial lesions. We studied electrocardiograms, echocardiograms, blood pressure, and heart rate in 14 adult male rats after bilateral microinjection into the NTS of stabilized substance P conjugated to the toxin saporin and compared the variables in five sham control rats and in five animals with toxin injected outside the NTS. Only injection of toxin into the NTS led to increased lability of arterial blood pressure, a sign of baroreflex interruption. Two animals treated with toxin died suddenly. All animals engaged in normal activity until, in two, rapid development of asystole and death over 6-8 min. Cardiac function when examined by echocardiography was normal, but pathologic examination of the heart revealed diffuse microscopic areas of acute coagulation necrosis in the myocardium in five animals, focal subacute necrosis in two animals, and both changes in one animal. This study supports the hypothesis that NTS lesions interrupting the baroreflex may induce cardiac arrhythmias and myocardial changes similar to those seen in humans with central lesions and may lead to sudden cardiac death.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Arrhythmias, Cardiac / chemically induced
  • Arrhythmias, Cardiac / complications
  • Arrhythmias, Cardiac / mortality
  • Arrhythmias, Cardiac / physiopathology*
  • Baroreflex*
  • Blood Pressure
  • Death, Sudden, Cardiac / etiology*
  • Death, Sudden, Cardiac / pathology
  • Disease Models, Animal
  • Echocardiography, Doppler, Pulsed
  • Electrocardiography
  • Heart / innervation*
  • Heart / physiopathology*
  • Heart Rate
  • Male
  • Microinjections
  • Myocardium / pathology*
  • Necrosis
  • Rats
  • Receptors, Neurokinin-1 / metabolism
  • Ribosome Inactivating Proteins, Type 1 / administration & dosage
  • Saporins
  • Solitary Nucleus / metabolism
  • Solitary Nucleus / physiopathology*
  • Substance P / administration & dosage
  • Substance P / analogs & derivatives
  • Sympathetic Nervous System / physiopathology*
  • Time Factors

Substances

  • Receptors, Neurokinin-1
  • Ribosome Inactivating Proteins, Type 1
  • substance P-saporin
  • Substance P
  • Saporins