Abstract
We evaluated B memory responses in healthy adult volunteers who received one oral dose of live-attenuated Shigella flexneri 2a vaccine. LPS-specific B(M) cells increased from a median of 0 at baseline to 20 spot forming cells (SFC)/10(6) expanded cells following vaccination (p=0.008). A strong correlation was found between post-vaccination anti-LPS B(M) cell counts and peak serum anti-LPS IgG titers (rs=0.95, p=0.0003). Increases in B(M) specific for IpaB approaching significance were also observed. In sum, oral vaccination with live-attenuated S. flexneri 2a elicits B(M) cells to LPS and IpaB, suggesting that B(M) responses to Shigella antigens should be further studied as a suitable surrogate of protection in shigellosis.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Administration, Oral
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Adolescent
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Adult
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Antibodies, Bacterial / biosynthesis*
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Antibodies, Bacterial / blood
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Antibodies, Bacterial / immunology
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Antibody Specificity
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Antigens, Bacterial / genetics
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Antigens, Bacterial / immunology
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B-Lymphocytes / immunology*
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Bacterial Vaccines / administration & dosage
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Bacterial Vaccines / immunology*
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Dysentery, Bacillary / prevention & control
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Female
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Humans
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Immunization
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Immunologic Memory*
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Lipopolysaccharides / immunology*
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Male
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Middle Aged
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Plasmids
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Shigella flexneri / genetics
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Shigella flexneri / immunology*
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Vaccines, Attenuated / administration & dosage
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Vaccines, Attenuated / immunology
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Volunteers
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Young Adult
Substances
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Antibodies, Bacterial
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Antigens, Bacterial
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Bacterial Vaccines
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Lipopolysaccharides
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Vaccines, Attenuated