Structural probing of Zn(II), Cd(II) and Hg(II) binding to human ubiquitin

Chem Commun (Camb). 2008 Dec 7:(45):5960-2. doi: 10.1039/b813463d. Epub 2008 Oct 9.

Abstract

A structural investigation performed on adducts of human ubiquitin with group-12 metal ions reveals common preferential anchoring sites, the most populated one being His68; at higher metal ion concentration a second and a third site, close to the N-terminus of the protein, become populated and promote a polymorphic transition from orthorhombic to cubic form; Glu16 and Glu18, involved in the latter metal binding, undergo a remarkable displacement from their position in native ubiquitin; the aggregate stereochemistry appears to be driven by the clustering of deshielded backbone hydrogen-bond patches, and metal ions foster this process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cadmium / chemistry*
  • Cadmium / metabolism
  • Humans
  • Mercury / chemistry*
  • Mercury / metabolism
  • Molecular Conformation
  • Protein Structure, Tertiary
  • Ubiquitin / chemistry*
  • Ubiquitin / metabolism
  • Zinc / chemistry*
  • Zinc / metabolism

Substances

  • Ubiquitin
  • Cadmium
  • Mercury
  • Zinc