Abstract
Objective:
To evaluation the effect of different mucosal vaccination pathway on hantavirus with the recombinant E. coli heat-labile enterotoxin B subunit (rLTB) as adjuvant.
Methods:
The rLTB was expressed and purified. Take the inactivated hantavirus strain 84Fli as vaccine, and immunized C57 BL/6 mice through intranasal, oral and vaginal respectively. Specific IgG and sectory IgA were detected by ELISA in serum, and vaginal washing samples respectively.
Results:
The rLTB was efficiently expressed under the induction of lactose, identified by western blotting and GM-1 binding experiment. The vaccination through intranasal, oral and vaginal, can induce IgG and sectory IgA response.
Conclusion:
Inactivated hantavirus can produce mucosal immune response with rLTB as adjuvants through intranasal, oral and vaginal vaccination respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / administration & dosage*
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Adjuvants, Immunologic / genetics
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Animals
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Antibodies, Viral / blood
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Bacterial Toxins / administration & dosage
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Bacterial Toxins / genetics
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Bacterial Toxins / immunology*
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Enterotoxins / administration & dosage
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Enterotoxins / genetics
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Enterotoxins / immunology*
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Escherichia coli Proteins / administration & dosage
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / immunology*
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Female
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Hantavirus Infections / immunology*
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Hantavirus Infections / virology
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Humans
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Immunity, Mucosal
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Immunoglobulin A / blood
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Immunoglobulin G / blood
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Mice
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Mice, Inbred C57BL
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Orthohantavirus / genetics
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Orthohantavirus / immunology*
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Random Allocation
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Viral Vaccines / administration & dosage
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Viral Vaccines / genetics
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Viral Vaccines / immunology*
Substances
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Adjuvants, Immunologic
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Antibodies, Viral
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Bacterial Toxins
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Enterotoxins
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Escherichia coli Proteins
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Immunoglobulin A
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Immunoglobulin G
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Viral Vaccines
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heat-labile enterotoxin, E coli