Early development of human hematopoietic and acquired immune systems in new born NOD/Scid/Jak3null mice intrahepatic engrafted with cord blood-derived CD34 + cells

Int J Hematol. 2008 Dec;88(5):476-482. doi: 10.1007/s12185-008-0215-z. Epub 2008 Nov 28.

Abstract

An animal model in which the human immune system can be reconstituted is necessary to study acquired immunity in vivo. We report here a novel model, the NOD/SCID/JAK3(null) mouse, for the human immune system's development. Newborn mice transplanted with human cord blood CD34(+) cells intrahepatically, developed human T and B cells, and myeloid and plasmacytoid dendritic cells. The T and B cells had a naïve to memory phenotype, and included plasma cells. The human acquired immune system can be reconstituted from CD34(+) cells in NOD/SCID/JAK3(null) mice. This model is a powerful tool for the study of human immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn*
  • Antigens, CD34*
  • Cord Blood Stem Cell Transplantation / methods
  • Fetal Blood / cytology
  • Fetal Blood / immunology*
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / immunology*
  • Humans
  • Immunologic Memory / physiology
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / immunology*
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Models, Immunological*
  • Transplantation, Heterologous

Substances

  • Antigens, CD34