Objective: To evaluate the effects of four agents including cyclophosphamide, thalidomide, total glucosides of peony (TGP) and simvastatin on cell-proliferation and endothelin-1 secretion of human endothelial cells (ECs).
Methods: EA. hy926 cells were cultured until confluence was achieved, then incubated separately for 24 h, 48 h and 72 h with various concentration of these four agents. The proliferation of ECs was detected with 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromide (MTT). The amount of endothelin-1 in supernatants of ECs was determined with enzyme linked immunosorbent assay.
Results: (1) Cyclophosphamide, TGP and simvastatin inhibited ECs proliferation in a dose dependent manner at 72 h (P <0.05). (2) Cyclophosphamide (50-2000 micromol/L) and simvastatin (5-10 micromol/L) decreased endothelin-1 secretion but not in dose dependent manner.
Conclusion: Cyclophosphamide, TGP and simvastatin can inhibit ECs proliferation. Cyclophosphamide and simvastatin can decrease endothelin-1 secretion of human ECs.