Convenient TLC-based Identification Test for the Crude Drug "Pogostemoni Herba"

Yakugaku Zasshi. 2008 Dec;128(12):1833-7. doi: 10.1248/yakushi.128.1833.

Abstract

TLC and HPLC were used to identify possible chemical markers for evaluating the quality of the crude drug "Pogostemoni herba" (aerial part of Pogostemon cablin), which is a component of Kampo medicines. In addition to the reported patchouli alcohol and 2-hydroxy-6-methyl-3-(4-methylpentanoyl)-4-pyrone, three phenylethanoids were isolated from this plant material for the first time: acteoside, isoacteoside, and crenatoside. The usefulness of these compounds as indicators of the crude commercial drug under various TLC conditions was examined, and patchouli alcohol was found to give a definite spot with a reproducible Rf value. Therefore, we propose TLC of the methanol (MeOH) extract using patchouli alcohol as a marker as a convenient method for identifying the crude drug Pogostemoni herba.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Caffeic Acids / isolation & purification*
  • Chromatography, Thin Layer / methods*
  • Drugs, Chinese Herbal / chemistry*
  • Glucosides / isolation & purification*
  • Lamiaceae / chemistry*
  • Methanol
  • Phenols / isolation & purification*
  • Sesquiterpenes / isolation & purification*

Substances

  • Caffeic Acids
  • Drugs, Chinese Herbal
  • Glucosides
  • Phenols
  • Sesquiterpenes
  • crenatoside
  • acteoside
  • isoacteoside
  • patchouli alcohol
  • Methanol