Abstract
Cells from the human monocytic cell-line THP1 were incubated prior to activation with IFN-gamma or LPS with varying amounts of p24, the main product of the HIV gag gene and the major component of the virus core. The IFN-gamma-dependent increase of mRNA for HLA-DR and for the heavy chain of cytochrome b was markedly decreased by p24 but not by gp120. This effect was abrogated by anti-p24 antibodies. On the other hand, preincubation of THP1 cells with p24 did not affect the accumulation of the LPS-dependent mRNA for TNF alpha and IL1-beta. These results indicate that p24 at concentrations similar to those found in the serum of HIV-infected individuals specifically affects IFN-gamma-induced activation markers.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Blotting, Northern
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Cell Line
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Cytochrome b Group / genetics*
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Gene Expression / drug effects
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Gene Products, gag / pharmacology*
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HIV Core Protein p24
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HIV-1 / immunology*
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HLA-DR Antigens / genetics*
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Humans
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Interferon-gamma / antagonists & inhibitors*
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Interleukin-1 / genetics
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Interleukin-1 / metabolism
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Lipopolysaccharides / pharmacology
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Macrophage Activation
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Monocytes / microbiology
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Monocytes / physiology*
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RNA, Messenger / genetics
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / metabolism
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Viral Core Proteins / pharmacology*
Substances
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Cytochrome b Group
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Gene Products, gag
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HIV Core Protein p24
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HLA-DR Antigens
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Interleukin-1
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Lipopolysaccharides
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Viral Core Proteins
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Interferon-gamma