Tick-borne pathogens in Thailand can cause diseases that result in productivity and economic losses in the livestock sector as well as cause debilitating illnesses in humans and their companion animals. With the advent of molecular techniques, accurate identification of tick-borne pathogens and precise diagnosis of disease is now available. This literature review summarizes the various tick-borne pathogens that have been isolated from ticks and their vertebrate hosts in Thailand, covering those protozoa, rickettsiae, bacteria and viruses most responsible for human and veterinary disease with particular emphasis on those that have been characterized molecularly.