Abstract
This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.
Publication types
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Research Support, Non-U.S. Gov't
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Video-Audio Media
MeSH terms
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Animals
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Cell Culture Techniques / methods*
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Collagen*
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Drug Combinations
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Embryo, Mammalian
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Embryonic Stem Cells / cytology*
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Fibroblasts / cytology*
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Humans
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Laminin*
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Mice
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Proteoglycans*
Substances
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Drug Combinations
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Laminin
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Proteoglycans
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matrigel
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Collagen