Abstract
A simple high-performance liquid chromatography (HPLC) method to determine the content of betulinic acid (BA) in rat plasma collected at different times (0-8 h) after oral administration of Orthosiphon stamineus leaf extract was developed. The features of the assay include protein precipitation using acetonitrile and isocratic elution using reverse phase C-18 column with ultraviolet (UV) detection. The recovery of BA from plasma varied from 98.4 to 102.5%. The R.S.D of intra- and inter-day precision from rat plasma ranged from 4.2 to 9.8%. The maximum concentration of BA in the plasma was 1.2 +/- 0.3 microg/ml at 1 h after oral administration of the extract.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Betulinic Acid
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Calibration
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Chromatography, High Pressure Liquid
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Data Interpretation, Statistical
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Indicators and Reagents
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Mice
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Orthosiphon / chemistry*
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Pentacyclic Triterpenes
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Pharmaceutic Aids / chemistry
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Plant Extracts / chemistry
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Plant Extracts / pharmacology
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Plant Leaves
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Povidone / chemistry
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Rats
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Rats, Sprague-Dawley
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Reference Standards
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Spectrometry, Mass, Electrospray Ionization
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Triterpenes / analysis
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Triterpenes / blood
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Triterpenes / pharmacokinetics*
Substances
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Indicators and Reagents
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Pentacyclic Triterpenes
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Pharmaceutic Aids
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Plant Extracts
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Triterpenes
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Povidone
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Betulinic Acid