ICP-MS-based multiplex profiling of glycoproteins using lectins conjugated to lanthanide-chelating polymers

J Proteome Res. 2009 Feb;8(2):443-9. doi: 10.1021/pr800645r.

Abstract

Lectins have been increasingly important in the study of glycoproteins. Here, we report a glycoprofiling method based on the covalent attachment of metal-chelating polymers to lectins for use in an ICP-MS-based assay. The labeled lectins are able to distinguish between glycoproteins covalently attached to a microtiter plate and their binding can be directly quantified by ICP-MS. Since each conjugate contains a different lanthanide, the assays can be conducted in a single or multiplex fashion, and may be readily elaborated to many different assay formats.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Chelating Agents / chemistry
  • Glycoproteins / chemistry*
  • Lanthanoid Series Elements / chemistry*
  • Lectins / chemistry*
  • Mass Spectrometry / methods*
  • Models, Molecular
  • Molecular Structure
  • Polymers / chemistry*
  • Protein Array Analysis / methods*

Substances

  • Chelating Agents
  • Glycoproteins
  • Lanthanoid Series Elements
  • Lectins
  • Polymers