Abstract
Apolipoprotein M (apoM) has been suggested to play a role in reverse cholesterol transport. Here we studied the influence of liver X-receptor (LXR) agonist on the transcriptional regulation of apoM. Studies were performed in murine liver and intestinal mucosal cells in vivo and in human intestinal Caco-2 cells in vitro. The expression of apoM was analyzed by quantitative real time PCR, and compared to well-established LXR target genes. Mice fed with TO901317 for six days showed a downregulation of apoM and apoAI in the liver to 40 % and 60 % respectively and an upregulation of Cyp7A1 to 280 %. In the small intestine, however, apoM and apoAI were upregulated by 30-60 % and ABCA1 by 250-430 %. In Caco-2 cells TO901317 caused a 60 % upregulation and the natural LXR agonist 22-hydroxycholesterol a 40 % upregulation of apoM. Possible causes for the differential effects in liver and intestine are discussed.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Apolipoproteins / metabolism*
-
Apolipoproteins M
-
Caco-2 Cells
-
DNA-Binding Proteins / drug effects*
-
DNA-Binding Proteins / metabolism*
-
Gene Expression Regulation / drug effects
-
Gene Expression Regulation / physiology
-
Humans
-
Hydrocarbons, Fluorinated / administration & dosage*
-
Intestinal Mucosa / metabolism*
-
Intestines / drug effects
-
Lipocalins
-
Liver / drug effects
-
Liver / metabolism*
-
Liver X Receptors
-
Organ Specificity / drug effects
-
Organ Specificity / physiology
-
Orphan Nuclear Receptors
-
Receptors, Cytoplasmic and Nuclear / drug effects*
-
Receptors, Cytoplasmic and Nuclear / metabolism*
-
Signal Transduction / drug effects
-
Signal Transduction / physiology*
-
Sulfonamides / administration & dosage*
Substances
-
APOM protein, human
-
Apolipoproteins
-
Apolipoproteins M
-
DNA-Binding Proteins
-
Hydrocarbons, Fluorinated
-
Lipocalins
-
Liver X Receptors
-
Orphan Nuclear Receptors
-
Receptors, Cytoplasmic and Nuclear
-
Sulfonamides
-
T0901317