Single-photon-counting detector for increased sensitivity in two-photon laser scanning microscopy

Opt Lett. 2008 Dec 15;33(24):2895-7. doi: 10.1364/ol.33.002895.

Abstract

We present the use and characterization of a photon-counting detector for increased sensitivity at low signal levels in fluorescence laser scanning microscopy (LSM). Conventional LSM photomultiplier tube detectors utilize analog current integration and thus suffer from excessive noise at low signal levels, introduced during current measurement. In this Letter we describe the implementation of a fast single-photon-counting (SPC) detector on a conventional two-photon laser scanning microscope and detail its use in imaging low fluorescence intensities. We show that for a low photon flux, the SPC detector is shot-noise limited and thus provides increased detection sensitivity compared with analog current integration.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Humans
  • Islets of Langerhans / pathology
  • Lasers*
  • Microscopy, Confocal / instrumentation
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods*
  • NAD / chemistry
  • NADP / chemistry
  • Optics and Photonics
  • Photons*
  • Scattering, Radiation

Substances

  • NAD
  • NADP