Differentiation of Mycobacterium tuberculosis and Mycobacterium bovis BCG by a polymerase chain reaction assay

B B Plikaytis; K D Eisenach; J T Crawford; T M Shinnick

doi:10.1016/0890-8508(91)90043-j

Differentiation of Mycobacterium tuberculosis and Mycobacterium bovis BCG by a polymerase chain reaction assay

Mol Cell Probes. 1991 Jun;5(3):215-9. doi: 10.1016/0890-8508(91)90043-j.

Authors

B B Plikaytis¹, K D Eisenach, J T Crawford, T M Shinnick

Affiliation

¹ Division of Bacterial Diseases, Centers for Disease Control, Atlanta GA 30333.

PMID: 1908051
DOI: 10.1016/0890-8508(91)90043-j

Abstract

Mycobacterium tuberculosis and Mycobacterium bovis are closely related species which carry different numbers of the repetitive DNA element IS6110. A polymerase chain reaction assay was developed to assess the copy number of IS6110 in a strain and thereby differentiate these two important human pathogens.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Base Sequence
Genes, Bacterial
Molecular Sequence Data
Mycobacterium bovis / classification*
Mycobacterium bovis / genetics
Mycobacterium tuberculosis / classification*
Mycobacterium tuberculosis / genetics
Polymerase Chain Reaction*
Repetitive Sequences, Nucleic Acid