1H, 13C, and 15N resonance assignments of murine amelogenin, an enamel biomineralization protein

Biomol NMR Assign. 2008 Jun;2(1):89-91. doi: 10.1007/s12104-008-9092-x.

Abstract

Amelogenin is the predominant matrix protein in developing dental enamel. Making extensive use of residue specific 15N-labeled amino acids samples, the majority of the main and side chain resonances for murine amelogenin were assigned in 2% aqueous acetic acid at pH 3.0.

Keywords: Amelogenin; Biomineralization; Enamel; Polyproline type II structure; Unfolded protein.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amelogenin / chemistry*
  • Amino Acid Sequence
  • Animals
  • Carbon Isotopes / chemistry
  • Dental Enamel / chemistry*
  • Magnetic Resonance Spectroscopy / methods*
  • Mice
  • Minerals / chemistry*
  • Molecular Sequence Data
  • Molecular Weight
  • Nitrogen Isotopes / chemistry
  • Protons

Substances

  • Amelogenin
  • Carbon Isotopes
  • Minerals
  • Nitrogen Isotopes
  • Protons