The allelic distribution of 15 short tandem repeats (STRs) loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA) were tested in 497 unrelated Yunnan Han individuals by the AmpFl STR Identifiler kit. No deviation from Hardy-Weinberg equilibrium was observed except D7S820 locus treated by Bonferroni correction. Genetic parameters of forensic interests were calculated and the genetic differences between Yunnan Han and other eight populations were analyzed.