Abstract
Purpose:
To determine the effect of keratocyte-derived MT1-MMP on calf pulmonary artery endothelial cell (CPAE) proliferation and migration.
Methods:
Keratocyte lines were generated from MT1-MMP knockout (KO) and wild type (WT) mice. WT keratocytes were transfected with WT or mutant MT1-MMP DNAs (DeltaTC or E240A). The effect of keratocyte-conditioned media on CPAE proliferation and migration was assayed.
Results:
KO keratocyte conditioned media resulted in the greatest increase of CPAE cell proliferation (190.5+/-6.0%; p<0.01). WT keratocyte conditioned media showed higher CPAE proliferation (155.4+/-3.6%) than WT/MT1-MMP-transfected keratocytes (119.7+/-2.2%; p<0.001). Migration assays confirmed these findings.
Conclusions:
Keratocyte-derived MT1-MMP has anti-angiogenic effects in CPAE cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Angiogenesis Inhibitors / deficiency*
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Angiogenesis Inhibitors / genetics
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Angiogenesis Inhibitors / metabolism*
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Angiogenesis Inhibitors / pharmacology
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Animals
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Cattle
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Cell Line, Transformed
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Cell Movement / drug effects
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Cell Proliferation / drug effects
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Cells, Cultured
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Cornea / cytology
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Cornea / enzymology*
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Culture Media, Conditioned / pharmacology
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Endothelial Cells / cytology
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Endothelial Cells / physiology*
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Matrix Metalloproteinase 14 / deficiency*
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Matrix Metalloproteinase 14 / genetics
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Matrix Metalloproteinase 14 / metabolism*
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Matrix Metalloproteinase 14 / pharmacology
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Mice
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Mice, Knockout
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Mutation
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Pulmonary Artery / cytology*
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Transfection
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Up-Regulation
Substances
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Angiogenesis Inhibitors
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Culture Media, Conditioned
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Matrix Metalloproteinase 14