Abstract
Sequencing upstream from the vanA gene of enterococcal plasmid pIP816 that confers Vm resistance revealed the presence of an ORF which could code for a protein of 322 aa designated VanH. Extensive aa similarity was detected between VanH and 2-hydroxycarboxylic acid dehydrogenase. We discuss possible roles for VanH in the synthesis of a novel type of peptidoglycan precursors with lower affinity for Vm.
MeSH terms
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Alcohol Oxidoreductases / chemistry*
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Amino Acid Sequence
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics
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Base Sequence
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Carboxylic Acids / metabolism
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Drug Resistance, Microbial
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Enterococcus faecalis / drug effects
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Enterococcus faecalis / genetics*
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Molecular Sequence Data
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Open Reading Frames / genetics
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Peptidoglycan / metabolism
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Plasmids / genetics
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Sequence Alignment
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Vancomycin / metabolism
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Vancomycin / pharmacology
Substances
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Bacterial Proteins
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Carboxylic Acids
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Peptidoglycan
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VanH protein, Enterococcus faecium
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Vancomycin
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Alcohol Oxidoreductases
Associated data
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GENBANK/M64053
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GENBANK/M64054
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GENBANK/M64055
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GENBANK/M64056
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GENBANK/M64057
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GENBANK/M64058
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GENBANK/M64059
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GENBANK/M64060
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GENBANK/M64304
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GENBANK/S53112