Failure of serum transforming growth factor-beta (TGF-beta1) as a biomarker of radiographic osteoarthritis at the knee and hip: a cross-sectional analysis in the Johnston County Osteoarthritis Project

Osteoarthritis Cartilage. 2009 Jun;17(6):772-6. doi: 10.1016/j.joca.2008.11.010. Epub 2008 Nov 21.

Abstract

Purpose: To assess associations between serum transforming growth factor-beta (TGF-beta1) and radiographic knee and hip osteoarthritis (rOA) in African American (AA) and White men and women.

Methods: Baseline data from 330 participants in the Johnston County Osteoarthritis Project were used in the analysis. Radiographs were scored with the Kellgren-Lawrence scale and rOA defined as grade> or =2. Individual radiographic features (IRFs) were rated 0-3. TGF-beta1 was measured using a sandwich enzyme-linked immunosorbent assay (ELISA). General linear models were used to estimate associations between lnTGF-beta1 and rOA presence, laterality or severity, and IRF presence and severity, adjusting for age, gender, race and body mass index. Interactions by race and gender were considered significant at P<0.1.

Results: Mean lnTGF-beta1 levels were higher among AAs compared to Whites, and among women compared to men (P<0.009). Mean lnTGF-beta1 levels were higher in those with knee osteophytes (OST), but this association was not significant after adjustment. There were no other significant differences in mean lnTGF-beta1 levels by presence, laterality, or severity of knee or hip rOA or IRFs. No race or gender interactions were identified, although a borderline significant association between lnTGF-beta1 and knee OST was seen among AAs (P<0.06).

Conclusions: Although serum TGF-beta1 varied by race and gender and several rOA variables, there were no independent significant associations with presence, laterality, or severity of knee or hip rOA by K-L grade or IRFs, suggesting that serum TGF-beta1 is unlikely to be useful as a stand-alone biomarker in OA studies. A possible association between TGF-beta1 and OST in AAs cannot be excluded.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / blood
  • Cross-Sectional Studies
  • Female
  • Hip Joint / diagnostic imaging
  • Hip Joint / metabolism*
  • Humans
  • Knee Joint / diagnostic imaging
  • Knee Joint / metabolism*
  • Male
  • Middle Aged
  • Osteoarthritis, Hip / blood*
  • Osteoarthritis, Hip / diagnostic imaging
  • Osteoarthritis, Hip / ethnology
  • Osteoarthritis, Knee / blood*
  • Osteoarthritis, Knee / diagnostic imaging
  • Osteoarthritis, Knee / ethnology
  • Radiography
  • Transforming Growth Factor beta1 / blood*
  • Weight-Bearing

Substances

  • Biomarkers
  • Transforming Growth Factor beta1