Oligonucleotide-mediated retroviral RNase H activation leads to reduced HIV-1 titer in patient-derived plasma

AIDS. 2009 Jan 14;23(2):213-21. doi: 10.1097/QAD.0b013e32831c5480.

Abstract

Background: The retroviral RNase H is essential for viral replication. This component has not yet been extensively studied for antiviral therapy. It can be activated by an oligodeoxynucleotide (ODN) resulting in self-destruction of the virions.

Objective: To examine antiviral potential of ODN in clinical samples using plasma of HIV-1-infected patients.

Design: Plasma of 19 HIV-1-infected patients from Zurich and 10 HIV-1 isolates from Africa and drug-resistant strains were processed for ex-vivo treatment.

Methods: Cell-free virions were treated with ODN in the plasma and HIV RNA was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, infectivity of the treated virions was tested on primary human peripheral blood mononuclear cells.

Results: Cell-free virions in plasma contained significantly less intact HIV RNA upon treatment with ODN (P = 0.0004), and their infectivity was decreased 52-fold (P = 0.0004). In 39% of the Zurich samples, infectivity was reduced more than 10-fold, in 33% more than 100-fold, and in 28% more than 1000-fold. Also, the isolates from Africa exhibited a 63-fold reduction in infectivity (P = 0.0069) with 80% of the isolates responding more than 10-fold, 40% more than 100-fold, and 10% more than 1000-fold.

Conclusion: Significant reduction of plasma HIV RNA levels and infectivity of treated virions was achieved on the basis of induced self-destruction of HIV observed with clinical samples. Reduction of viral load ex vivo was designed as model for potential effects in vivo. Premature activation rather than inhibition of a viral enzyme could be a model strategy for future antiretroviral control.

MeSH terms

  • Base Sequence
  • Cells, Cultured
  • DNA, Viral / genetics
  • Drug Resistance, Viral
  • Enzyme Activation / genetics
  • Genetic Therapy / methods
  • HIV Infections / virology*
  • HIV-1 / drug effects
  • HIV-1 / enzymology
  • HIV-1 / isolation & purification*
  • HIV-1 / pathogenicity
  • Humans
  • Leukocytes, Mononuclear / virology
  • Molecular Sequence Data
  • Oligonucleotides, Antisense / genetics
  • Oligonucleotides, Antisense / pharmacology*
  • RNA, Viral / blood
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Ribonuclease H / metabolism*
  • Viral Load
  • Virion / pathogenicity
  • Virus Inactivation

Substances

  • DNA, Viral
  • Oligonucleotides, Antisense
  • RNA, Viral
  • Ribonuclease H