Abstract
In this paper we report a new method that permitted for the first time to selectively track a polysaccharide-based hydrogel on bone tissue explants, several weeks after its implantation. The hydrogel, which was developed for bone healing and tissue engineering, was labelled with a ruthenium complex and implanted into rabbit bone defects in order to investigate its in vivo degradation. 1, 2, 3 and 8 weeks after surgery, the bone explants were analyzed by synchrotron X-ray microfluorescence, infrared mapping spectroscopy, scanning electron microscopy, and optical microscopy after histological coloration. The results showed that the labelled polysaccharide-based hydrogel was likely to undergo phagocytosis that seemed to occur from the edge to the center of the implantation site up to at least the 8th week.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Absorbable Implants*
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Animals
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Biocompatible Materials / metabolism*
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Bone and Bones / drug effects
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Bone and Bones / metabolism*
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Calcium Phosphates / metabolism
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Cell Line
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Cell Survival / drug effects
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Cells, Cultured
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Ceramics / metabolism
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Chondrocytes / cytology
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Chondrocytes / drug effects
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Cross-Linking Reagents / pharmacology
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Femur / pathology
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Femur / ultrastructure
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Humans
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Hydrogel, Polyethylene Glycol Dimethacrylate / metabolism*
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Hypromellose Derivatives
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Methylcellulose / analogs & derivatives*
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Methylcellulose / chemistry
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Methylcellulose / metabolism
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N-Acetylneuraminic Acid / chemistry
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N-Acetylneuraminic Acid / metabolism
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Osteogenesis / drug effects
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Prosthesis Implantation
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Rabbits
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Ruthenium / metabolism*
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Time Factors
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Tissue Engineering*
Substances
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Biocompatible Materials
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Calcium Phosphates
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Cross-Linking Reagents
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Hydrogel, Polyethylene Glycol Dimethacrylate
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Hypromellose Derivatives
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Ruthenium
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Methylcellulose
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calcium phosphate
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N-Acetylneuraminic Acid