Abstract
A novel protein kinase homologue (KNS1) has been identified in Saccharomyces cerevisiae. KNS1 contains an open reading frame of 720 codons. The carboxy-terminal portion of the predicted protein sequence is similar to that of many other protein kinases, exhibiting 36% identity to the cdc2 gene product of Schizosaccharomyces pombe and 34% identity to the CDC28 gene product of S. cerevisiae. Deletion mutations were constructed in the KNS1 gene. kns1 mutants grow at the same rate as wild-type cells using several different carbon sources. They mate at normal efficiencies, and they sporulate successfully. No defects were found in entry into or exit from stationary phase. Thus, the KNS1 gene is not essential for cell growth and a variety of other cellular processes in yeast.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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CDC2 Protein Kinase / genetics
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Chromosome Deletion
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Fungal Proteins / genetics*
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Kinetics
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Molecular Sequence Data
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Multigene Family / genetics*
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Mutation / genetics
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Open Reading Frames / genetics
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Protein Kinases / genetics*
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Protein Serine-Threonine Kinases
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Recombinant Fusion Proteins / biosynthesis
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Restriction Mapping
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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beta-Galactosidase / genetics
Substances
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Fungal Proteins
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KNS1 protein, S cerevisiae
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Protein Kinases
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Protein Serine-Threonine Kinases
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CDC2 Protein Kinase
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beta-Galactosidase
Associated data
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GENBANK/M36980
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GENBANK/S55162
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GENBANK/S55712
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GENBANK/X56671
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GENBANK/X56672
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GENBANK/X63982
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GENBANK/X63983
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GENBANK/X63984
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GENBANK/X63985
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GENBANK/X63986
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GENBANK/X63987