Abstract
In this study, 68 group A streptococcus (GAS) isolates associated with two outbreaks of acute glomerulonephritis (AGN) in China were analyzed by emm typing. A total of 11 different emm types were identified. Analysis of emm type distribution suggested that AGN outbreaks in two counties were caused by emm60.1- and emm63.0-type GAS. These two types were further characterized by pulsed-field gel electrophoresis, multilocus sequence typing, sof sequence typing, and PCR-based identification of streptococcal pyrogenic exotoxin A, B, and C (speA, speB, and speC) genes. In antimicrobial susceptibility tests, all outbreak strains were resistant to erythromycin and tetracycline, and the rates of resistance of nonoutbreak strains to the two antibiotics were 63.6% and 90.9%. This study is also the first to report a nephritogenic M63 GAS strain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Animals
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Anti-Bacterial Agents / pharmacology
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Antigens, Bacterial / genetics
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Toxins / genetics
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Bacterial Typing Techniques
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Carrier Proteins / genetics
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Child
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Child, Preschool
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China / epidemiology
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Cluster Analysis
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DNA Fingerprinting
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Disease Outbreaks*
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Electrophoresis, Gel, Pulsed-Field
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Genotype
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Glomerulonephritis / epidemiology*
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Glomerulonephritis / microbiology*
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Humans
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Sequence Analysis, DNA
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Streptococcal Infections / epidemiology*
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Streptococcal Infections / microbiology*
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Streptococcus pyogenes / classification*
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Streptococcus pyogenes / genetics*
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Streptococcus pyogenes / isolation & purification
Substances
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Anti-Bacterial Agents
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Bacterial Toxins
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Carrier Proteins
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DNA, Bacterial
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streptococcal M protein
Associated data
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GENBANK/EF489042
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GENBANK/EF489043
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GENBANK/EF494746
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GENBANK/EF494747