Paracrine signaling by platelet-derived growth factor-CC promotes tumor growth by recruitment of cancer-associated fibroblasts

Cancer Res. 2009 Jan 1;69(1):369-78. doi: 10.1158/0008-5472.CAN-08-2724.

Abstract

Cancer results from the concerted performance of malignant cells and stromal cells. Cell types populating the microenvironment are enlisted by the tumor to secrete a host of growth-promoting cues, thus upholding tumor initiation and progression. Platelet-derived growth factors (PDGF) support the formation of a prominent tumor stromal compartment by as of yet unidentified molecular effectors. Whereas PDGF-CC induces fibroblast reactivity and fibrosis in a range of tissues, little is known about the function of PDGF-CC in shaping the tumor-stroma interplay. Herein, we present evidence for a paracrine signaling network involving PDGF-CC and PDGF receptor-alpha in malignant melanoma. Expression of PDGFC in a mouse model accelerated tumor growth through recruitment and activation of different subsets of cancer-associated fibroblasts. In seeking the molecular identity of the supporting factors provided by cancer-associated fibroblasts, we made use of antibody arrays and an in vivo coinjection model to identify osteopontin as the effector of the augmented tumor growth induced by PDGF-CC. In conclusion, we establish paracrine signaling by PDGF-CC as a potential drug target to reduce stromal support in malignant melanoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Growth Processes / physiology
  • Cell Line, Tumor
  • Fibroblasts / metabolism
  • Fibroblasts / pathology
  • Humans
  • Lymphokines / biosynthesis
  • Lymphokines / genetics
  • Lymphokines / metabolism*
  • Melanoma / genetics
  • Melanoma / metabolism
  • Melanoma / pathology
  • Melanoma, Experimental / genetics
  • Melanoma, Experimental / metabolism*
  • Melanoma, Experimental / pathology*
  • Mice
  • Mice, Inbred C57BL
  • NIH 3T3 Cells
  • Osteopontin / biosynthesis
  • Platelet-Derived Growth Factor / biosynthesis
  • Platelet-Derived Growth Factor / genetics
  • Platelet-Derived Growth Factor / metabolism*
  • Receptor, Platelet-Derived Growth Factor alpha / biosynthesis
  • Signal Transduction
  • Skin Neoplasms / genetics
  • Skin Neoplasms / metabolism*
  • Skin Neoplasms / pathology*
  • Stromal Cells / metabolism
  • Stromal Cells / pathology

Substances

  • Lymphokines
  • Platelet-Derived Growth Factor
  • platelet-derived growth factor C
  • Osteopontin
  • Receptor, Platelet-Derived Growth Factor alpha