Abstract
The under-representation of HLA antigens in human tumors is usually associated with a poor prognosis. In this report, the expression of HLA class I genes in human colorectal carcinomas was studied using HLA-A and HLA-B locus-specific probes. Over 50% of the colorectal carcinomas studied showed a reduction in the amount of steady state HLA class I mRNA. For some carcinomas, non-coordinated regulation of the HLA-A and HLA-B genes was observed. The mechanism of HLA suppression was investigated and is most likely due to the presence of transcriptional regulatory elements.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blotting, Northern
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Blotting, Southern
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Carcinoembryonic Antigen / biosynthesis
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Cell Line
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Chloramphenicol O-Acetyltransferase / biosynthesis
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Colonic Neoplasms / genetics
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Colonic Neoplasms / immunology*
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Down-Regulation
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Female
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HLA-A Antigens / genetics
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HLA-A Antigens / metabolism
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HLA-B Antigens / genetics
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HLA-B Antigens / metabolism
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Histocompatibility Antigens / genetics
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Histocompatibility Antigens / metabolism*
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Humans
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Interferon-gamma / pharmacology
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Male
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Methylation
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Proto-Oncogene Proteins c-myc / biosynthesis
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RNA, Messenger / biosynthesis
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Rectal Neoplasms / genetics
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Rectal Neoplasms / immunology*
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Transfection
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Tumor Cells, Cultured
Substances
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Carcinoembryonic Antigen
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HLA-A Antigens
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HLA-B Antigens
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Histocompatibility Antigens
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Proto-Oncogene Proteins c-myc
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RNA, Messenger
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Interferon-gamma
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Chloramphenicol O-Acetyltransferase