Tailoring the P450 monooxygenase gene for FR-008/candicidin biosynthesis

Appl Environ Microbiol. 2009 Mar;75(6):1778-81. doi: 10.1128/AEM.00859-08. Epub 2009 Jan 9.

Abstract

Tailoring steps are often important for the activity of mature antibiotics. Here, we report that novel decarboxylated FR-008/candicidin derivatives were obtained from the P450 monooxygenase gene fscP mutant of Streptomyces sp. strain FR-008. The toxicity of decarboxylated FR-008/candicidin derivatives has been shown to be greatly reduced compared to that of wild-type FR-008/candicidin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / biosynthesis*
  • Anti-Bacterial Agents / toxicity
  • Candicidin / biosynthesis*
  • Candicidin / toxicity
  • Erythrocytes / drug effects
  • Gene Deletion
  • Genes, Bacterial
  • Metabolic Networks and Pathways
  • Mixed Function Oxygenases / genetics
  • Mixed Function Oxygenases / metabolism*
  • Molecular Structure
  • Rabbits
  • Streptomyces / genetics
  • Streptomyces / metabolism*

Substances

  • Anti-Bacterial Agents
  • Candicidin
  • Mixed Function Oxygenases