Abstract
Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM). Transfected cells with GH-EYFP fusion protein and rab3B-ECFP fusion protein were observed under CLSM, which showed the colocalization of EYFP-GH and ECFP-rab3B in the budding configuration of secretory process. These structural and functional images of rab3B imply the incorporation of rab3B in the secretion of GH through porosome.
MeSH terms
-
Animals
-
Bacterial Proteins / metabolism*
-
Cell Extracts
-
Cell Line
-
Green Fluorescent Proteins / metabolism*
-
Growth Hormone / metabolism*
-
Luminescent Proteins / metabolism*
-
Microscopy, Confocal
-
Pituitary Gland / cytology*
-
Pituitary Gland / metabolism*
-
Rats
-
Recombinant Fusion Proteins / metabolism*
-
Transfection*
-
rab3 GTP-Binding Proteins / metabolism*
Substances
-
Bacterial Proteins
-
Cell Extracts
-
Luminescent Proteins
-
Recombinant Fusion Proteins
-
enhanced cyan fluorescent protein
-
yellow fluorescent protein, Bacteria
-
Green Fluorescent Proteins
-
Growth Hormone
-
Rab3b protein, rat
-
rab3 GTP-Binding Proteins