Abstract
The differentiation of T cells along different lineages is central to the control of immunity. Here we have used a conditional gene knockout system to delete PKC lambda/iota selectively in activated T cells. With this system we have demonstrated that PKC lambda/iota is necessary for T-helper cell (Th2) cytokine production and optimal T-cell proliferation and allergic airway inflammation in vivo. Our data demonstrate that the activation of the transcription factors nuclear factor of activated T cells and NF-kappaB is impaired in PKC lambda/iota-deficient activated T cells. In addition, we present genetic knockout evidence in ex vivo experiments with primary T cells that PKC lambda/iota is critical for the control of cell polarity during T-cell activation. Therefore PKC lambda/iota emerges as a critical regulator of Th 2 activation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Differentiation
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Cell Polarity
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Cell Proliferation
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Cytokines / metabolism
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Hypersensitivity / enzymology*
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Hypersensitivity / immunology*
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Immunoglobulin E / blood
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Inflammation / enzymology*
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Inflammation / immunology
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Isoenzymes / deficiency*
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Isoenzymes / metabolism
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Lymphocyte Activation
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Mice
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Mice, Knockout
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Ovalbumin
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Protein Kinase C / deficiency*
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Protein Kinase C / metabolism
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Respiratory System / enzymology
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Respiratory System / immunology
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Respiratory System / pathology*
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Th2 Cells / cytology
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Th2 Cells / enzymology*
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Th2 Cells / immunology*
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Th2 Cells / metabolism
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Transcription Factors / metabolism
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Up-Regulation
Substances
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Cytokines
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Isoenzymes
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Transcription Factors
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Immunoglobulin E
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Ovalbumin
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Protein Kinase C
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protein kinase C lambda