Abstract
The need for reporter lines able to faithfully track Th17 cells in vivo has become an issue of exceptional importance. To address this, we generated a mouse strain in which Cre recombinase is expressed from the IL-17F promoter. Crossing the IL-17F-Cre allele to a conditional enhanced yellow fluorescent protein (EYFP) reporter mouse yielded the IL-17F-Cre(EYFP) strain, in which IL-17F expression is twinned with EYFP in live IL-17F-expressing cells. Although we demonstrate that IL-17F expression is restricted to CD4(+) T cells during experimental autoimmune encephalomyelitis, IL-17F-Cre(EYFP) CD8 T cells robustly expressed IL-17F in response to TGF-beta, IL-6, and IL-23. Fate mapping of IL-17F-expressing reporter T cells revealed a significant down-regulation of Th17 cytokines after homeostatic expansion in RAG1-deficient animals. Despite this loss of effector phenotype, committed Th17 cells were resistant to Foxp3 expression in vitro or in vivo. Thus, the IL-17F-Cre strain furthers our understanding of Th17 biology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adoptive Transfer
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Animals
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Bacterial Proteins / genetics*
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CD8-Positive T-Lymphocytes / cytology
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CD8-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / pathology
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Cell Differentiation / genetics*
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Cell Differentiation / immunology*
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Cells, Cultured
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Encephalomyelitis, Autoimmune, Experimental / immunology
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Encephalomyelitis, Autoimmune, Experimental / metabolism
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Encephalomyelitis, Autoimmune, Experimental / pathology
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Gene Expression Regulation / immunology
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Genes, Reporter / immunology*
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Humans
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Immunophenotyping
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Integrases / genetics*
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Interleukin-17 / biosynthesis
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Interleukin-17 / genetics*
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Interleukin-17 / physiology
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Luminescent Proteins / genetics*
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Mice
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Mice, Inbred C57BL
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Mice, Inbred DBA
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Mice, Transgenic
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Proteins / genetics
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RNA, Untranslated
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T-Lymphocytes, Regulatory / immunology*
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T-Lymphocytes, Regulatory / metabolism*
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T-Lymphocytes, Regulatory / pathology
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T-Lymphocytes, Regulatory / transplantation
Substances
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Bacterial Proteins
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Gt(ROSA)26Sor non-coding RNA, mouse
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Interleukin-17
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Luminescent Proteins
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Proteins
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RNA, Untranslated
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yellow fluorescent protein, Bacteria
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Cre recombinase
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Integrases