Abstract
The use of Cyan Fluorescent Proteins, with a distinctive lifetime signature, opens up new alternatives to track and semi-quantify the relative expression of proteins in vivo using a single excitation source and emission channel.
MeSH terms
-
Animals
-
Cricetinae
-
Directed Molecular Evolution
-
Genetic Techniques*
-
Green Fluorescent Proteins / metabolism*
-
HeLa Cells
-
Humans
-
Hydrogen-Ion Concentration
-
Microscopy, Fluorescence / instrumentation*
-
Microscopy, Fluorescence / methods*
-
Mutation
-
Protein Engineering / methods
-
Temperature
-
Time Factors
Substances
-
Cyan Fluorescent Protein
-
Green Fluorescent Proteins