Effects of cholesterol- or 7-ketocholesterol-containing liposomes on colony-forming ability of cultured cells

FEBS Lett. 1991 Sep 23;290(1-2):171-2. doi: 10.1016/0014-5793(91)81252-4.

Abstract

Experiments with cultured Chinese hamster cells showed that incubation of the cells with (phosphatidylcholine + cholesterol + 7-ketocholesterol)-containing liposomes (4:3:1 by weight) during two hours led to a decrease in the colony-forming ability of cells down to zero, while (phosphatidylcholine + cholesterol)-containing liposomes (1:1 by weight) reduce this parameter by 90%. Furthermore, the cholesterol-containing liposomes (without 7-ketocholesterol) induce a decrease in the number of the maximal-site colonies accompanied by the corresponding increase in the number of the middle-size colonies.

MeSH terms

  • Animals
  • Cell Division*
  • Cell Line
  • Cholesterol / chemistry*
  • Cricetinae
  • Cricetulus
  • In Vitro Techniques
  • Ketocholesterols / chemistry*
  • Liposomes
  • Membrane Lipids / chemistry
  • Membrane Lipids / physiology*

Substances

  • Ketocholesterols
  • Liposomes
  • Membrane Lipids
  • Cholesterol
  • 7-ketocholesterol