Applications of optical resonance to biological imaging and label-free protein microarrays

Annu Int Conf IEEE Eng Med Biol Soc. 2008:2008:2643. doi: 10.1109/IEMBS.2008.4649744.

Abstract

We present biological imaging and sensing methods based on optical resonance and interference. In fluorescence microscopy, our nanoscale imaging capability sheds light onto conformational changes of DNA, DNA-protein complexes and polymer coatings on a solid surface. Interference measurements on a layered substrate yield a label-free sensing platform for protein binding in a high-throughput micro-array format.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biosensing Techniques
  • Electrochemistry / instrumentation
  • Fluorescence
  • Fluorescent Dyes
  • Humans
  • Microscopy, Fluorescence / instrumentation*
  • Microscopy, Fluorescence / methods*
  • Nucleic Acid Hybridization
  • Optics and Photonics / instrumentation*
  • Optics and Photonics / methods*
  • Protein Array Analysis / instrumentation*
  • Protein Array Analysis / methods*
  • Proteins / chemistry*

Substances

  • Fluorescent Dyes
  • Proteins