SNP genotyping using the Sequenom MassARRAY iPLEX platform

Curr Protoc Hum Genet. 2009 Jan:Chapter 2:Unit 2.12. doi: 10.1002/0471142905.hg0212s60.

Abstract

The method for SNP genotyping described in this unit is based on the commercially available Sequenom MassARRAY platform. The assay consists of an initial locus-specific PCR reaction, followed by single base extension using mass-modified dideoxynucleotide terminators of an oligonucleotide primer which anneals immediately upstream of the polymorphic site of interest. Using MALDI-TOF mass spectrometry, the distinct mass of the extended primer identifies the SNP allele.

MeSH terms

  • Alleles
  • DNA Primers / genetics
  • Genotype*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymerase Chain Reaction / methods
  • Polymorphism, Single Nucleotide*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • DNA Primers