Detection of human immunodeficiency virus (HIV) type 1 M184V and K103N minority variants in patients with primary HIV infection

Antimicrob Agents Chemother. 2009 Apr;53(4):1670-2. doi: 10.1128/AAC.01494-08. Epub 2009 Jan 26.

Abstract

We used an allele-specific real-time PCR assay to explore the presence of K103N and M184V minority species among primary human immunodeficiency virus (HIV) infections and their potential influence in HIV transmission. Thirty randomly chosen antiretroviral drug-naive patients lacking both the K103N and the M184V mutations as determined by conventional sequencing methods were studied, and K103N and M184V viral minority species were found in three (10%) and four (11%) patients, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Drug Resistance, Viral
  • HIV Infections / drug therapy
  • HIV Infections / virology*
  • HIV Reverse Transcriptase / genetics*
  • HIV-1 / genetics*
  • Humans
  • Molecular Sequence Data
  • Mutation*
  • RNA, Viral / blood

Substances

  • RNA, Viral
  • reverse transcriptase, Human immunodeficiency virus 1
  • HIV Reverse Transcriptase

Associated data

  • GENBANK/EU375800
  • GENBANK/EU375801
  • GENBANK/EU906882
  • GENBANK/EU906883
  • GENBANK/EU906884
  • GENBANK/EU906885
  • GENBANK/EU906886
  • GENBANK/EU906887
  • GENBANK/EU906888
  • GENBANK/EU906889
  • GENBANK/EU906890
  • GENBANK/EU906891
  • GENBANK/EU906892
  • GENBANK/EU906893
  • GENBANK/EU906894
  • GENBANK/EU906895
  • GENBANK/EU906896
  • GENBANK/EU906897
  • GENBANK/EU906898
  • GENBANK/EU906899
  • GENBANK/EU906900
  • GENBANK/EU906901
  • GENBANK/EU906902
  • GENBANK/EU906903
  • GENBANK/EU906904
  • GENBANK/EU906905
  • GENBANK/EU906906
  • GENBANK/EU906907