Abstract
Seven in one blow: The efficient formation of mixed disulfides on the thiol-rich fusion protein A followed by subsequent intein cleavage gave the fragment B with all seven cysteines protected against oxidation. The native chemical ligation of B with synthetic glycopeptide thioesters provides glycoproteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Endoribonucleases / biosynthesis
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Endoribonucleases / chemical synthesis*
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Endoribonucleases / chemistry
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Glycopeptides / biosynthesis
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Glycopeptides / chemical synthesis*
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Glycopeptides / chemistry
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Glycoproteins / biosynthesis
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Glycoproteins / chemical synthesis*
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Glycoproteins / chemistry
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Ligation
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Oxidation-Reduction
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Protein Engineering
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Protein Folding
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Protein Renaturation
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / chemical synthesis
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Recombinant Fusion Proteins / chemistry
Substances
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Glycopeptides
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Glycoproteins
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Recombinant Fusion Proteins
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Endoribonucleases
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ribonuclease C