Determination of carbadox-related residues in swine liver by gas chromatography/mass spectrometry with ion trap detection

J Assoc Off Anal Chem. 1991 Jul-Aug;74(4):611-8.

Abstract

The ion trap detector (ITD), in combination with a capillary gas chromatograph and under chemical ionization conditions, offers sufficient sensitivity to determine carbadox-related residues as the methyl ester derivative of quinoxaline-2-carboxylic acid at 3 micrograms/kg or higher in porcine liver. A tetradeuterated internal standard of QME effectively compensates for losses incurred during sample preparation. The method produced mean levels of 3.3 (+/- 0.5), 5.5 (+/- 0.8), and 10.1 (+/- 0.9) micrograms/kg for liver fortified at 3, 5, and 10 micrograms/kg. When applied to analysis of samples containing incurred residues of 14C-carbadox at the low microgram/kg level, results were comparable to those obtained by reverse isotope dilution analysis.

MeSH terms

  • Animals
  • Carbadox / analysis*
  • Carbadox / pharmacokinetics
  • Carbon Radioisotopes
  • Chromatography, Thin Layer
  • Deuterium
  • Drug Residues / analysis*
  • Drug Residues / pharmacokinetics
  • Gas Chromatography-Mass Spectrometry / methods
  • Liver / chemistry*
  • Liver / metabolism
  • Microchemistry / methods
  • Reference Standards
  • Swine

Substances

  • Carbon Radioisotopes
  • Deuterium
  • Carbadox