Protection of guinea pigs and swine by a recombinant adenovirus expressing O serotype of foot-and-mouth disease virus whole capsid and 3C protease

Zengjun Lu; Huifang Bao; Yimei Cao; Pu Sun; Jianhun Guo; Pinghua Li; Xingwen Bai; Yingli Chen; Baoxia Xie; Dong Li; Zaixin Liu; Qingge Xie

doi:10.1016/j.vaccine.2008.09.066

Protection of guinea pigs and swine by a recombinant adenovirus expressing O serotype of foot-and-mouth disease virus whole capsid and 3C protease

Vaccine. 2008 Dec 19:26 Suppl 6:G48-53. doi: 10.1016/j.vaccine.2008.09.066.

Authors

Zengjun Lu¹, Huifang Bao, Yimei Cao, Pu Sun, Jianhun Guo, Pinghua Li, Xingwen Bai, Yingli Chen, Baoxia Xie, Dong Li, Zaixin Liu, Qingge Xie

Affiliation

¹ Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, Xujiaping No. 1, Yanchangpu, Lanzhou, Gansu 730046, People's Republic of China.

PMID: 19178894
DOI: 10.1016/j.vaccine.2008.09.066

Abstract

Two recombinant adenoviruses were constructed expressing foot-and-mouth disease virus (FMDV) capsid and 3C/3CD proteins in replicative deficient human adenovirus type 5 vector. Guinea pigs vaccinated with 1-3 x 10(8)TCID(50) Ad-P12x3C recombinant adenovirus were completely protected against 10,000GID(50) homologous virulent FMDV challenge 25 days post vaccination (dpv). Ad-P12x3CD vaccinated guinea pigs were only partially protected. Swine were vaccinated once with 1x10(9)TCID(50) Ad-P12x3C hybrid virus and challenged 28 days later. Three of four vaccinated swine were completely protected against 200 pig 50% infectious doses (ID(50)) of homologous FMDV challenge, and vaccinated pigs developed specific cellular and humoral immune responses. The immune effect of Ad-P12x3C in swine further indicated that the recombinant adenovirus was highly efficient in transferring the foreign gene. This approach may thus be a very hopeful tool for developing FMD live virus vector vaccine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3C Viral Proteases
Adenoviridae* / genetics
Animals
Antibodies, Viral / blood
Capsid* / immunology
Capsid* / metabolism
Cysteine Endopeptidases* / genetics
Cysteine Endopeptidases* / immunology
Cysteine Endopeptidases* / metabolism
Foot-and-Mouth Disease / immunology
Foot-and-Mouth Disease / prevention & control*
Foot-and-Mouth Disease / virology
Foot-and-Mouth Disease Virus / classification
Foot-and-Mouth Disease Virus / immunology*
Guinea Pigs
Recombinant Proteins* / administration & dosage
Recombinant Proteins* / immunology
Recombinant Proteins* / metabolism
Serotyping
Swine
Swine Diseases / immunology
Swine Diseases / prevention & control*
Swine Diseases / virology
Vaccination / veterinary
Viral Proteins* / genetics
Viral Proteins* / immunology
Viral Proteins* / metabolism
Viral Vaccines / administration & dosage
Viral Vaccines / immunology

Substances

Antibodies, Viral
Recombinant Proteins
Viral Proteins
Viral Vaccines
Cysteine Endopeptidases
3C Viral Proteases