Yeast RNA polymerase II subunit RPB9 is essential for growth at temperature extremes

J Biol Chem. 1991 Oct 5;266(28):19053-5.

Abstract

The Saccharomyces cerevisiae RNA polymerase II subunit gene RPB9 was isolated and sequenced. RPB9 is a single copy gene on chromosome VII. The RPB9 sequence predicts a protein of 122 amino acids with a molecular mass of 14,200 Da. The yeast RPB9 subunit is similar in size and sequence to a protein encoded by DNA adjacent to the suppressor of the Hairy Wing gene in Drosophila melanogaster. Deletion of the RPB9 gene produced cells that were heat- and cold-sensitive. The RPB9 subunit, like the previously described RNA polymerase II subunit RPB4, is not essential for synthesis of mRNA, but is required for normal cell growth over a wide temperature range.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping
  • DNA, Fungal
  • Genes, Fungal
  • Molecular Sequence Data
  • RNA Polymerase II / genetics
  • RNA Polymerase II / physiology*
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Sequence Alignment
  • Temperature

Substances

  • DNA, Fungal
  • RNA Polymerase II

Associated data

  • GENBANK/M72403
  • GENBANK/M72404
  • GENBANK/M72405
  • GENBANK/M72406
  • GENBANK/M72407
  • GENBANK/M72408
  • GENBANK/M73060
  • GENBANK/S58194
  • GENBANK/S58196
  • GENBANK/S58286