Abstract
How PHDs achieve specificity: trans-4-prolyl hydroxylation of the transcription factor HIF occurs with stereochemical retention. Substrate-analogue studies show how the von Hippel Lindau tumor suppressor protein (pVHL) and the oxygen-sensing hydroxylases (PHDs) achieve specificity for hydroxyprolyl/prolyl residues for the C(4)-exo/endo prolyl conformations, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biosensing Techniques
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit / chemistry*
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Hypoxia-Inducible Factor 1, alpha Subunit / metabolism
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Hypoxia-Inducible Factor-Proline Dioxygenases
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Oxygen / metabolism*
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Procollagen-Proline Dioxygenase / metabolism*
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Protein Conformation
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Stereoisomerism
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Von Hippel-Lindau Tumor Suppressor Protein / metabolism
Substances
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Hypoxia-Inducible Factor 1, alpha Subunit
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EGLN1 protein, human
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Procollagen-Proline Dioxygenase
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Hypoxia-Inducible Factor-Proline Dioxygenases
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Von Hippel-Lindau Tumor Suppressor Protein
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Oxygen