Abstract
Separation of ethyl acetate fractionation of Dracocephalum peregrinum afforded three new flavonoid glucosides (1-3), and a new cyanogenic glucoside (4). Their structures were elucidated based on HR-electron spray ionization (ESI)-MS, EI-MS, UV, IR, 1D-, and 2D-NMR data. 1-4 were tested in vitro for their antiinflammatory activity against the RAW 264.7, 293 cells. Among the compounds tested, 1-4 shown good antiinflammatory activity at 100 mug/ml by the measurement of nitric oxide (NO) in lipopolysaccharide (LPS) activated macrophages. But only 2 and 3 shown weak antiinflammatory activity at 100 mug/ml during the nuclear factor (NF)-kappaB activation assay.
MeSH terms
-
Anti-Inflammatory Agents / chemistry
-
Anti-Inflammatory Agents / isolation & purification
-
Anti-Inflammatory Agents / pharmacology
-
Carbohydrate Conformation
-
Cell Line
-
Dimethyl Sulfoxide
-
Flavonoids / chemistry*
-
Flavonoids / isolation & purification
-
Flavonoids / pharmacology
-
Glycosides / chemistry
-
Humans
-
Lamiaceae / chemistry*
-
Lipopolysaccharides / pharmacology
-
Macrophages / drug effects
-
Magnetic Resonance Spectroscopy
-
NF-kappa B / metabolism
-
Nitric Oxide / metabolism
-
Solvents
-
Spectrometry, Mass, Electrospray Ionization
-
Spectrophotometry, Infrared
Substances
-
Anti-Inflammatory Agents
-
Flavonoids
-
Glycosides
-
Lipopolysaccharides
-
NF-kappa B
-
Solvents
-
Nitric Oxide
-
Dimethyl Sulfoxide