Combined hyperspectral and spectral domain optical coherence tomography microscope for noninvasive hemodynamic imaging

Opt Lett. 2009 Feb 1;34(3):289-91. doi: 10.1364/ol.34.000289.

Abstract

We have combined hyperspectral imaging with spectral domain optical coherence tomography (SDOCT) to noninvasively image changes in hemoglobin saturation, blood flow, microvessel morphology, and sheer rate on the vessel wall with tumor growth. Changes in these hemodynamic variables were measured over 24 h in dorsal skin fold window chamber tumors. There was a strong correlation between volumetric flow and hemoglobin saturation (rho=0.89, p=9x10(-6), N=15) and a moderate correlation between shear rate on the vessel wall and hemoglobin saturation (rho=0.56, p=0.03, N=15).

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Blood Flow Velocity
  • Cell Line, Tumor
  • Computer-Aided Design
  • Equipment Design
  • Equipment Failure Analysis
  • Hemoglobins / analysis*
  • Mice
  • Neovascularization, Pathologic / diagnosis
  • Neovascularization, Pathologic / pathology*
  • Neovascularization, Pathologic / physiopathology*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Skin Neoplasms / diagnosis
  • Skin Neoplasms / pathology*
  • Skin Neoplasms / physiopathology*
  • Spectrum Analysis / instrumentation*
  • Spectrum Analysis / methods
  • Systems Integration
  • Tomography, Optical Coherence / instrumentation*
  • Tomography, Optical Coherence / methods

Substances

  • Hemoglobins