This paper reports the occurrence of parasitism by the Oriental eye-fluke, Philophthalmus gralli in ostriches reared in a commercial flock from the State of Minas Gerais State, Southeastern Brazil. The two affected birds presented lacrimation, inflammatory reaction and loss of body condition. After physical examination the birds revealed, 17 and 15 trematodes each, only in the right eyes. The economic impact of this parasite on Brazilian ostrich industry is still undetermined, requiring further studies.