Previous studies have documented the ability of bovine milk to inhibit lymphocyte proliferation in response to mitogens. It is not known whether inhibition of lymphocyte proliferation is mediated through the action of monocytes. To address this question, we examined the ability of bovine skim milk and whey to affect monocyte function with emphasis on expression of major histocompatibility class II antigens and production of interleukin-1 by monocytes. Data showed that expression of major histocompatibility complex class II molecules and production of interleukin-1 by monocytes were not altered when monocytes were cultured in the presence of bovine skim milk or whey. Thus, it is unlikely that the suppressive effect of milk on lymphocyte proliferation could be mediated through alterations in the expression of major histocompatibility class II molecules or in production of interleukin-1 by monocytes. The role of other monocyte antigens or secretory products, however, should also be evaluated.