Abstract
Four echinomycin antibiotics were isolated from the culture broth of a marine streptomycete, and their structures were determined by a combination of chemical and spectroscopic analyses. Antibiotic activities were measured against drug-resistant and biofilm-forming strains of Staphylococcus aureus and Enterococcus faecalis. Minimum inhibitory concentrations ranging from 0.01 microM to greater than 14 microM clearly defined structure-activity relationships for antibiotic potency. Echinomycin was the most active compound with a MIC of 0.03 microM against methicillin-resistant S. aureus and 0.01 microM against biofilm-forming E. faecalis.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Anti-Bacterial Agents / chemistry*
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Anti-Bacterial Agents / isolation & purification
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Anti-Bacterial Agents / pharmacology
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Biofilms / drug effects*
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Biofilms / growth & development
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Drug Resistance, Multiple, Bacterial / drug effects*
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Drug Resistance, Multiple, Bacterial / physiology
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Echinomycin / chemistry*
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Echinomycin / isolation & purification
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Echinomycin / pharmacology
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Enterococcus faecalis / drug effects*
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Enterococcus faecalis / growth & development
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HCT116 Cells
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Humans
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Methicillin-Resistant Staphylococcus aureus / drug effects
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Methicillin-Resistant Staphylococcus aureus / growth & development
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Microbial Sensitivity Tests / methods
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Spectrometry, Mass, Electrospray Ionization
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / growth & development
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Structure-Activity Relationship
Substances
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Anti-Bacterial Agents
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Echinomycin