Reliability of the low dose synacthen test in children undergoing pituitary function testing

J Pediatr Endocrinol Metab. 2008 Dec;21(12):1129-32. doi: 10.1515/jpem.2008.21.12.1129.

Abstract

Background: There are few data in the paediatric literature on the normal cortisol response to stimulation during the low dose synacthen test (LDST) (1 microg).

Aim: To examine the cortisol responses in children, subsequently presumed to be normal, who had an LDST during anterior pituitary function tests (APFTs).

Methods: A retrospective review of results in children with short stature and normal growth hormone levels.

Results: Of 33 children tested, seven had suboptimal cortisol responses based on accepted criteria (peak <500 nmol/l)--a false positive rate of 21%. Only three of these children had a repeat LDST, which was normal in all cases. The peak cortisol response (median 633, range, 417-1052 nmol/l) was inversely correlated with age (r = -0.44, p < 0.05).

Conclusion: One in five tests did not meet normal criteria. This false positive rate (21%) should be borne in mind when interpreting synacthen tests to prevent overdiagnosis of adrenal insufficiency.

MeSH terms

  • Adolescent
  • Adrenal Insufficiency / blood
  • Adrenal Insufficiency / diagnosis*
  • Child
  • Child, Preschool
  • Cosyntropin*
  • Dose-Response Relationship, Drug
  • False Positive Reactions
  • Female
  • Humans
  • Hydrocortisone / blood*
  • Male
  • Pituitary Function Tests / methods*
  • Pituitary Gland, Anterior / physiology*
  • Reproducibility of Results
  • Retrospective Studies

Substances

  • Cosyntropin
  • adrenocorticotropin zinc
  • Hydrocortisone